Mapping Angiogenic Cells CD31 (PECAM1) and CD45 in PCa and BPH Biopsies

Molecular characterization of PCa and BPH using the angiogenic tissue markers CD31 and CD45 to differentiate between the two major types of prostate gland disorders is investigated. 8 clinically characterized biopsies were analyzed at the Histopathology Laboratory of the Ekiti State University Teaching Hospital; the tissue sections were processed for antigen retrieval and were labeled with anti-CD45 and anti-CD31 to map the location of angiogenic cells and vascularization in BPH and PCa. The results showed that PCa biopsies expressed embryonic stem cell status of CD31+/CD45+, while BPH showed CD31-/CD45+. This suggests the presence of migrating blood cells (vascularization) in PCa and the absence of such in BPH. It also confirms the role of platelets in endothelial activation that can result to cell proliferation and apoptosis in the prostate gland of either BPH or PCa diagnosed patients.   Key words: CD31, CD45, Prostate, Endothelioma, Vascularization, Angiogenesis