Cell growth curves for different cell lines and their relationship with biological activities

The cellular-growth curves for distinct cell lines (tumor and non-tumor) were established, evaluating the population of doubling time (DT) and maximum growth rate (µmax). These curves define the growth characteristics for each cell line; they allow determination of the best time range for evaluating the effects of some biological compounds. Subsequently, the biological activities of three ethanolic extracts of Phoradendron californicum were evaluated in these cells lines; two extracts were of Prosopis laevigata (Mesquite tree) from two distinct geographic zones, and the other one was of Quercus ilex (Encino/Oak tree). We also evaluated the cytotoxic activity and nitric oxide (NO) scavenging ability of these extracts. The results showed the RAW 264.7 cell-line had the highest growth rate in all experiments. For the rest of cell-lines, we found µmax in the following order: L929 > A549 > ARPE-19 ≥ 22Rv1; while the DT was in the order: RAW 264.7 > A549 ≥ L929 > ARPE-19 > 22Rv1. With respect to the anti-proliferative activity of the P. californicum ethanolic extracts, we observed that the most sensitive cell line was RAW 264.7 (with all three extracts), and the least sensitive was the ARPE-19 cell line; the first one was the fastest growing and the second was the one of the slowest. The anti-oxidative studies by NO scavenging were evaluated in RAW 264.7 cell-line, the best extracts were the ethanolic extracts of P. californicum from P. laevigata and were geographic zone no-dependent for these biological activities. Key words: Cellular-growth curve, doubling time (DT), P. californicum, anti-proliferative, NO scavenging.