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Assessing human-induced threatening factors to wildlife is the basis in determining and proposing appropriate conservation measures. The present study was conducted to assess impacts of human activities on wildlife in Gambella National Park, Southwest of Ethiopia, from October 2015 to March 2016, focusing on the case of Nile Lechwe (Kobus megaceros). The data in this study were gathered using questionnaires with structured interview and focus group discussion.  Data on the threatening factors were compared among villages using chi-square test in SPSS version 20 software. Out of the 384 respondents, 139(36.2), 51(13.2) 49(12.8), 43(11.2) and 41(10.7%) of them informed that agricultural investment, illegal hunting, overgrazing, rice cultivation in the area and habitat loss, respectively, were the most predominant human factors affecting Nile Lechwe. Thus suggests that agricultural expansion and illegal hunting are those human activities with the highest impact on the Nile Lechwe. Before designing and implementing any development investment particularly large scale agricultural expansions, the government and other stakeholders should give consideration and attention to the rapidly declining natural resource beside to the development. Therefore, designing appropriate eco-friendly management with options must be adopted to mediate the effects and minimize future impacts.       Key words: Agriculture, anthropogenic disturbance, habitats, Nile Lechwe, Gambella National park, wildlife.

Impacts of human activities on wildlife: The case of Nile Lechwe (Kobus megaceros) Gambella National Park, Southwest Ethiopia