Hairy roots production in Phyllanthus odontadenius Mll. Arg. by seedlings transformed with Agrobacterium rhizogenes A4RS/pHKN29

Phyllanthus odontadenius Mull. Arg is one of the most important medicinal plants in the genus Phyllanthus (Phyllanthaceae). It is used in different regions in the world for the treatment of various diseases for example malaria caused by Plasmodium falciparum causing millions deaths in tropical and subtropical regions. In this work, we experimented P. odontadenius seeds germination and seedlings infected by Agrobacterium rhizogenes A4RS/pHKN9. We obtained 36% of seeds that germinated for 84.6% of seedlings transformed which showed positive gfp activities. The number of hairy roots formed in infectious sites for seedlings infected with A. rhizogenes A4RS/pHKN29 is valued at 6.3±2.71 against 0 for control seedlings. Diagrams showed that mean length for the new-formed roots were 1.92±0.55 cm and 1.59±0.49 cm for hairy root.      Key words: Phyllanthus odontadenius, hairy roots, Agrobacterium rhizogenes, malaria, secondary metabolites.