Identification and quantification of caffeoylquinic acid derivatives in Cynara scolymus L. tablets and capsules

Cynara scolymus L. leaves, popularly known as globe artichoke, have cholagogue and choleretic properties which are attributed to caffeoylquinic acid derivatives. Although, there are already pharmacopoeial methods for quality control of artichoke dry leaf extract, it only shows the assessment of chlorogenic acid determination. This study aimed to adapt and validate a method for simultaneous determination of neochlorogenic, chlorogenic, cryptochlorogenic, cynarin and isochlorogenic acids A and C in tablets or capsules of globe artichoke leaf extract herbal medicines. Also, it evaluated the content of these caffeoylquinic acid derivatives in three commercial products and leaf extract. The validation considered the parameters of selectivity, precision, linearity, quantification and detection limits and accuracy. The method proved to be selective, precise and accurate. Commercial products and leaf extract had significantly different concentrations of standard compounds which may bring an impact on therapeutic outcomes. The method proposed was suitable for the identification and quantification of caffeoylquinic acid derivatives in tablets and capsules and could be used in quality control, ensuring the safety and efficacy of commercially prepared herbal medicines.   Key words: Herbal medicines, quality control, Cynara scolymus.