Anti-inflammatory effect and high performance liquid chromatography (HPLC) analysis of the EtOAc extract from Panzeria alaschanica

The aerial parts of Panzeria alaschanica are used to treat pelvic inflammation and chronic pelvic inflammation. The anti-inflammatory effects of P. alaschanica have been well known in folk medicine, which suggest that extracted components from P. alaschanica could potentially treat inflammatory disease. The aim of this study was to discover the bioactive compounds of P. alaschanica. To achieve this, ethyl acetate (EtOAc) extract was extracted from the aerial parts of P. alaschanica and the anti-inflammatory effects were investigated against inflammation induced by carrageenan and egg-albumin in rats. At the doses used, EtOAc extract (100, 200 and 400 mg/kg) showed significant and dose-dependent anti-inflammatory effects. According to the high performance liquid chromatography (HPLC) analysis of EtOAc extract, there are two major families of compounds in the plant, namely phenylpropanoids (verbascoside,5,6,7,4'-tetrahydroxyflavone-7-O-(6"-O-[E]-coumaroyl)-β glucopyranoside,5,6,7,4'-tetrahydroxyflavone-7-O-(6"-O-[E]-caffeoyl)-β-glucopyranoside) and flavonoids (7,4'-dimethoxy-apigenin, isoramnetin-3-O-rutinoside, isoramnetin-3-O-glucoside, kaempferol-3-O-glucoside, hyperin), which may explain the activity.   Key words: Panzeria alaschanica, anti-inflammatory, high performance liquid chromatography (HPLC) analysis, phenylpropanoids, flavonoids.