Cytotoxic effect of Plectranthus neochilus extracts in head and neck carcinoma cell lines | Zendy

Gabriel Álvares Borges | Zendy; Freitas Ferreira Juliana | Zendy; Silvia Taveira Elias | Zendy; Neves Silva Guerra Eliete | Zendy; Silveira D acirc maris | Zendy; Alberto Simeoni Luiz | Zendy

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Cytotoxic effect of Plectranthus neochilus extracts in head and neck carcinoma cell lines

Author(s) -

Gabriel Álvares Borges,

Freitas Ferreira Juliana,

Silvia Taveira Elias,

Neves Silva Guerra Eliete,

Silveira D acirc maris,

Alberto Simeoni Luiz

Publication year - 2016

Publication title -

african journal of pharmacy and pharmacology

Language(s) - English

Resource type - Journals

ISSN - 1996-0816

DOI - 10.5897/ajpp2015.4355

Subject(s) - hacat , cell culture , cytotoxicity , chemistry , head and neck squamous cell carcinoma , cytotoxic t cell , mtt assay , viability assay , hela , microbiology and biotechnology , carcinoma , cell , in vitro , cancer research , cancer , pathology , medicine , head and neck cancer , biology , biochemistry , genetics

Following a tendency of studying the potential effects of plant extracts to cancer, this study aimed to evaluate in vitro the cytotoxic activity of Plectranthus neochilus (PN) extracts and its fractions in head and neck squamous cell carcinoma (HNSCC) cell lines and assess their tumor specificity. MTT assay was conducted with two HNSCC cell lines, FaDu (hypopharynx carcinoma) and SCC-25 (tongue carcinoma), one keratinocyte (HaCat) and one fibroblast (L929) cell line. Two PN leaf crude extracts, one ethanolic (E) and one hexanic (H), and their nine fractions were tested. A dose-response curve was performed with hexane PNH fraction and a tumor specificity index (TSI) was calculated. For all cell lines studied, almost all extracts and fractions resulted in cell viability lower than 50%. Hexane and methanol PNH fractions were exceptions, causing a significantly low viability in SCC-25 (17.16 and 34.53%, respectively), but higher than 50% in FaDu, HaCat and L929. The dose-response curve with hexane PNH fraction resulted in a CC50 of 540.9 µg/mL for FaDu, 550 µg/mL for L929, 762.1 µg/mL for HaCat and 274.2 µg/mL for SCC-25. The TSI L929/FaDu was 1.01, HaCat/FaDu was 1.40, L929/SCC-25 was 2.00 and HaCat/SCC-25 was 2.77. TSIs indicate its specificity for tongue carcinoma cells, when compared to fibroblasts and keratinocytes. Key words: Head and neck, squamous cell carcinoma, extract, cytotoxicity, cell line.

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