Activity exerted by a benzamide derivative on injury by ischemia/reperfusion in an isolated heart model

Several studies indicate that some benzamide-derivatives have activity at cardiovascular level; nevertheless, there is scarce information about the effects exerted by the benzamide derivatives on cardiac injury caused by ischemia/reperfusion (I/R). In this experimental study, a new benzamide-derivative was synthetized with the objective of evaluating its activity on I/R in an I/R model of rat heart using the Langendorff technique. In addition, molecular mechanism involved in the effect induced by the benzamide derivative on perfusion pressure and coronary resistance was evaluated by measuring left ventricular pressure in the absence or presence of following compounds; nifedipine, indomethacin, propranolol and metoprolol. The results showed that the benzamide-derivative reduces infarct size compared with control. Other results showed that the benzamide derivative significantly increase the perfusion pressure and coronary resistance in isolated heart. Other data indicate that the benzamide-derivative increase left ventricular pressure in a dose-dependent manner (0.001 to 100 nM); however, this phenomenon was significantly inhibited by propranolol and metoprolol at a dose of 1 nM (p=0.05). In conclusion, these data suggest that cardioprotective activity of the benzamide-derivative is by stimulating catecholamine production and consequently induce changes in the left ventricular pressure levels. This phenomenon results in decrease of myocardial necrosis after ischemia and reperfusion. Key words: Heart, benzamide derivative, ischemia, propranolol, metoprolol.