Protective effect of 2,2,2-trichloroethanol on peptidoglycan-induced inflammation in murine macrophages | Zendy

Qingjun Pan | Zendy; Ling Ye | Zendy; Yaning Li | Zendy; Gang Wang | Zendy; Zhu Xuezhi | Zendy

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Protective effect of 2,2,2-trichloroethanol on peptidoglycan-induced inflammation in murine macrophages

Author(s) -

Qingjun Pan,

Ling Ye,

Yaning Li,

Gang Wang,

Zhu Xuezhi

Publication year - 2014

Publication title -

african journal of pharmacy and pharmacology

Language(s) - English

Resource type - Journals

ISSN - 1996-0816

DOI - 10.5897/ajpp2013.3682

Subject(s) - peptidoglycan , inflammation , macrophage , stimulation , chemistry , lipopolysaccharide , tumor necrosis factor alpha , flow cytometry , transfection , microbiology and biotechnology , biochemistry , in vitro , biology , immunology , enzyme , gene , endocrinology

The aim of this study was to investigate the effect of 2,2,2-trichloroethanol on peptidoglycan-induced murine macrophages inflammation. The effect of 2,2,2-trichloroethanol on the production of TNF-α and IL-6 by murine peritoneal macrophages with peptidoglycan-stimulation were investigated. Also, RAW264.7 macrophages transfected with a NF-κB luciferase reporter plasmid with the stimulation of peptidoglycan were used to test the effect of 2,2,2-trichloroethanol on NF-κB activity. Flow cytometry and Western blotting were used to check the expression levels of toll-like receptor 2 on treated RAW264.7 macrophages. 2,2,2-Trichloroethanol decreased the unregulated levels of IL-6 and TNF-α produced by the peritoneal macrophages stimulation with peptidoglycan. The NF-κB activities of the RAW264.7 macrophages stimulated by peptidoglycan were decreased. 2,2,2-Trichloroethanol decreased the peptidoglycan-induced murine macrophage inflammation response.

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