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TM 96 well microplate assays. Then eight known compounds (1-8), including five alkaloids (1-5), were isolated and characterized from this plant for the first time. All the compounds were tested for their antimicrobial and antitrypanosomal properties. Most of the pure compounds were also found to possess good antibacterial and antitrypanosomal properties. Berberine (MIC = 12.5 µg/ml), berberine chloroform (MIC = 25 µg/ml) and syringresinol (MIC = 12.5 µg/ml) were found to have anti-bacterial and anti-trypanosomal properties. The MIC values were determined for only active compounds.

Bioassay guided isolation and characterization of anti-microbial and anti-trypanosomal agents from Berberis glaucocarpa Stapf.