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The current study was designed to establish a method for examining whether the content of PEG-TCS could be determined by the Lowry method. The research applicability of the Lowry method on the determination of PEGylated trichosanthin (PEG-TCS) protein content was proposed for the first time. Procedures were designed to determine whether residual PEG interfered with the result of protein content determination, PEG (≤20 µg/ml) interference was not apparent. The interference of a PEGmodified group was analyzed by utlra violet spectrophotometric colorimetry, and the differences in the rates were 5.0 and 6.2%. The linearity, precision, and recovery were determined. There is good linear relationship from 30 to 70 μg/ml PEG-TCS. The experiment results provided sufficient data to establish a quality control method for the determination of PEG-TCS content, and they were conducive to the determination method establishment of other PEGylated proteins for non clinical experiment application.

Effects of PEGylation on the Lowry method for the content determination of trichosanthin