The protective effect of salidroside on oxidative stress injury induced by hydrogen peroxide in PC12 cell

The protective effect of salidroside (SD) on oxidative stress injury induced by hydrogen peroxide in PC12 cell was investigated. The PC 12 cell was cultured in RPMI-1640 medium supplemented with 10% newborn calf serum at 37°C in a 5% CO2 incubator. 40 μM of H2O2was conducted to establish an oxidative-stress injury model in vitro. The effect of SD was evaluated by using the model. The LDH release rates of PC12 cells in different treatment group was compared. The cell apoptosis was detected by using flow cytometry (FCM) and the cell proliferation by MTT assay. This paper found that pretreatment with SD could improve cell growth and proliferation in a concentration dependent manner, reduce the release of LDH and suppresses the H2O2-induced apoptosis of PC12 cells, Therefore, these results significantly suggested that SD possessed the protective activity of PC12 cells injury by H2O2 in vitro.   Key words: Salidroside, PC12 cell, oxidative stress, protective effect.