Antioxidant ability of squid ink polysaccharides as well as their protective effects on deoxyribonucleic acid DNA damage in vitro | Zendy

Ping Luo | Zendy

Open Access

Antioxidant ability of squid ink polysaccharides as well as their protective effects on deoxyribonucleic acid DNA damage in vitro

Author(s) -

Ping Luo

Publication year - 2013

Publication title -

african journal of pharmacy and pharmacology

Language(s) - English

Resource type - Journals

ISSN - 1996-0816

DOI - 10.5897/ajpp12.1335

Subject(s) - radical , antioxidant , dna damage , chemistry , polysaccharide , dna , dpph , squid , biochemistry , hydroxyl radical , biology , ecology

Squid ink polysaccharides (SIPS) were isolated from squid ink, a plentiful and multifunctional marine material, and were demonstrated to have amelioratory effects on cyclophosphamide-induced damage in internal organs of model animals by our previous reports. To further investigate the protective effects of SIPS on chemotherapeutic damage caused by cyclophosphamide, this paper evaluated the bioactivities of the marine polysaccharides with a view to their antioxidant ability and their protective effects on deoxyribonucleic acid (DNA) damage using tests such as hydroxyl radicals, reducing power assay and 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radicals, and pEGFP-N1 plasmid DNA treated with ultra violet (UV) plus H2O2. Data revealed that SIPS not only quenched hydroxyl radicals and 1,1-Diphenyl-2-picrylhydrazyl radicals, but showed its strong reducing power and also suppressed oxidative scission on plasmid DNA strand caused by hydroxyl radicals which originated from H2O2shown by UV. Key words: Squid ink polysaccharides (SIPS), antioxidant ability, deoxyribonucleic acid (DNA) damage.

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