Heat shock impact on the growth of Bacillus spp. (SUBB01) and its surveillance in minimal medium under shaking condition

Heat shock proteins (HSPs) are a family of proteins that are produced by all living organisms in response to exposure to stressful condition. The present research aims to study the physiology of Bacillus spp. (SUBB01) under aeration in diverse culture media and temperature at 47, 48, 49, 50, 52, 53 and 54°C. Bacterial growth was measured through enumeration of the viable and culturable growing cells that are capable of producing the colony-forming units (CFUs) on Luria–Bertani (LB) and nutrient agar (NA) plates for 24 to 48 h. The work also focused on the repercussion of Bacillus spp. against excessive temperatures (37, 45 and 50°C) in minimal media under shaking condition. Isolated microbes were demonstrated under a light microscope to observe their cellular morphology, shape and organization. There was high-temperature shock up to 50°C in both LB, NA, and agar media, with the presence of a demanding defense mechanism against heat shock in these bacterial cells. However, further molecular studies on the genetic makeup of such stress responses as well as the growth retrieval mechanisms of Bacillus spp. through the exogenous organic factors would be very important.   Key words: Heat stress, Bacillus spp. (SUBB01), viable cells, minimal media, bacterial growth.