Dual-path platform (DPP) and enzyme-linked immunosorbent assay (ELISA): Change the sequence of the tests does not change the number of positive dogs for canine visceral leishmaniasis | Zendy

Sara dos Santos Almeida | Zendy; Lobo Gomes Carla | Zendy; Costa Silva Elaynne | Zendy; Tolentino Rocha Brandao Sarah | Zendy; Patricia Aviz Wellida | Zendy; Pinheiro Larissa | Zendy; Oviedo Paciello Mauricio | Zendy; Sander Rodrigues Cangussu Alex | Zendy; Wagner de Souza Aguiar Raimundo | Zendy; Carlos Bertucci Barbosa Luiz | Zendy; Cordeiro Giunchetti Rodolfo | Zendy; Fernandes Viana Kelvinson | Zendy

Open Access

Dual-path platform (DPP) and enzyme-linked immunosorbent assay (ELISA): Change the sequence of the tests does not change the number of positive dogs for canine visceral leishmaniasis

Author(s) -

Sara dos Santos Almeida,

Lobo Gomes Carla,

Costa Silva Elaynne,

Tolentino Rocha Brandao Sarah,

Patricia Aviz Wellida,

Pinheiro Larissa,

Oviedo Paciello Mauricio,

Sander Rodrigues Cangussu Alex,

Wagner de Souza Aguiar Raimundo,

Carlos Bertucci Barbosa Luiz,

Cordeiro Giunchetti Rodolfo,

Fernandes Viana Kelvinson

Publication year - 2017

Publication title -

african journal of microbiology research

Language(s) - English

Resource type - Journals

ISSN - 1996-0808

DOI - 10.5897/ajmr2016.8300

Subject(s) - visceral leishmaniasis , christian ministry , leishmania , leishmaniasis , protocol (science) , medicine , immunology , virology , veterinary medicine , parasite hosting , pathology , alternative medicine , philosophy , theology , world wide web , computer science

The Brazilian Ministry of Health determined in 2012 that the official protocol for diagnosis of Canine Visceral Leishmaniasis (CVL) would be the Dual-Path Platform (DPP) for screening, followed by enzyme-linked immunosorbent assay (ELISA) for confirmation. This study evaluated serum samples from 426 dogs from a region in northern Brazil. All samples were tested according to the Official Protocol and the sequence inverting (ELISA followed DPP). Regardless of the protocol adopted, prevalence (14.7%) has not changed. The approach using ELISA followed by DPP state that, the number of positive animals in screening was higher compared to the official protocol. Screen the ELISA test could be more appropriate. Key words: Canine visceral leishmaniasis, Dual-Path Platform (DPP), enzyme-linked immunosorbent assay (ELISA), tocantins.

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