Molecular characterization of local strains of Bacillus thuringiensis in the North of Algeria
Author(s) -
M. Kebdani,
M. Mahdjoubi,
Ameur Chérif,
B. N. Gaouar,
Rebiahi S. A. and
Publication year - 2016
Publication title -
african journal of microbiology research
Language(s) - English
Resource type - Journals
ISSN - 1996-0808
DOI - 10.5897/ajmr2016.7946
Subject(s) - bacillus thuringiensis , bacillus cereus , bacillus subtilis , bacillus (shape) , microbiology and biotechnology , biology , bacillaceae , bacillales , polymerase chain reaction , strain (injury) , bacteria , gene , genetics , anatomy
The analysis of soil samples taken from orange groves located in four cities of Northern Algeria allowed the isolation and identification of 24 strains belonging to the group Bacillus cereus, based on their physiological, biochemical and molecular characters investigated. They were divided as follow: 12 strains of Bacillus thuringiensis, 1 strain of Bacillus pumilis, 4 strains of Bacillus cereus, 5 strains of Bacillus subtilis and 2 Bacillus mycoides strains. After the molecular characterization performed with the aid of polymerase chain reaction (PCR) analysis, it was confirmed from the parasporal bodies using a scanning electron microscope that the strains of the identified collection belong to the species, B. thuringiensis. Key words: Bacillus cereus, Bacillus thuringiensis, Bacillus pumilis, Bacillus subtilis, Bacillus mycoides, polymerase chain reaction, scanning EM.
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