Bioaccumulation of cadmium and lead by Shewanella oneidensis isolated from soil in Basra governorate, Iraq
Author(s) -
Jaafar Raghad,
Al Sulami Amin,
Al Taee Asaad
Publication year - 2016
Publication title -
african journal of microbiology research
Language(s) - English
Resource type - Journals
ISSN - 1996-0808
DOI - 10.5897/ajmr2016.7912
Subject(s) - shewanella oneidensis , bioaccumulation , cadmium , bacteria , chemistry , microbiology and biotechnology , shewanella , environmental chemistry , minimum inhibitory concentration , biology , antimicrobial , genetics , organic chemistry
In the present study heavy metals resistant bacteria were isolated from soil collected from Al-Zubair district in Basra governorate south of Iraq. On the basis of morphological, biochemical, 16S rRNA gene sequencing and phylogeny analysis, the isolates were authentically identified as Shewanella oneidensis in addition to Bacillus thuringiensis and Deinococcus radiodurans. The minimal inhibitory concentration (MIC) of isolates against cadmium (Cd) and lead (Pb) was determined on solid medium. S. oneidensis showed significant resistance to high concentrations of Cd (1000 mgl -1 ) and Pb (700 mgl -1 ). The bioaccumilation capabilities of S. oneidensis for Cd and Pb were monitored at different ion concentrations and contact times. The transmission electron microscope (TEM) study confirmed the accumulation of Cd and Pb by S. oneindensis causing morphological changes.
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