A simple nested polymerase chain reaction for differential identification of uspI and uspII genes encoding uropathogenic specific protein of uropathogenic Escherichia coli

The gene encoding a protein which was designated as uropathogenic specific protein was identified on pathogenicity island of uropathogenic Escherichia coli. The usp gene is mainly classified into two types (uspI and uspII) depending on the difference in DNA sequence at the 3’ region. A simple nested polymerase chain reaction assay was applied to differentiate uspI and uspII. The results indicate the presence of 22 uspI and 42 uspII genes positive isolates. This study will be useful for accuracy of uropathogenic specific protein pathogenicity island subtyping method for epidemiological study of uropathogenic Escherichia coli.