Development of a real-time PCR assay for the detection and quantification of Gluconacetobacter diazotrophicus in sugarcane grown under field conditions
Author(s) -
Marcos Paulo,
Luiz Simes-Arajo Jean,
Henrique Varial de Melo Leona,
de Souza Galisa Pricles,
Leal Lineu,
Ivo Baldani Jos,
Lcia Divan Baldani Vera
Publication year - 2014
Publication title -
african journal of microbiology research
Language(s) - Portuguese
Resource type - Journals
ISSN - 1996-0808
DOI - 10.5897/ajmr2014.6779
Subject(s) - bacteria , inoculation , biology , polymerase chain reaction , primer (cosmetics) , endophyte , microbial inoculant , diazotroph , real time polymerase chain reaction , microbiology and biotechnology , detection limit , botany , nitrogen fixation , chemistry , horticulture , chromatography , gene , biochemistry , genetics , organic chemistry
Soil Science department UFRRJ, BR 465, km 7, Seropédica RJ CEP: 23891-000, Brazil. Embrapa Agrobiologia, BR 465, km 7, Seropédica RJ CEP: 23891-000, Brazil. Plant Biotechnology department UFRJ, Avenida Carlos Chagas Filho, 373, Rio de Janeiro RJ CEP: 21941-902, Brazil. Ministry of Agrarian Development, Esplanada dos Ministérios, Bloco A/Ala Norte Brasília-DF CEP 70050-902, Brazil. Federal Institute of Alagoas Campus Murici, Rua Astolfo Lopes, s/n, , Murici AL Cep: 57.820-000, Brazil.
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