Antimicrobial assay of methanolic crude of Lonicera lanceolata | Zendy

Arifullah Khan | Zendy; Sadia Nazir | Zendy; Kamran Tahir | Zendy; Ul Haq Khan Zia | Zendy; Wu N | Zendy; Yasmeen Rehana | Zendy; Ali Irshad | Zendy

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Antimicrobial assay of methanolic crude of Lonicera lanceolata

Author(s) -

Arifullah Khan,

Sadia Nazir,

Kamran Tahir,

Ul Haq Khan Zia,

Wu N,

Yasmeen Rehana,

Ali Irshad

Publication year - 2014

Publication title -

african journal of microbiology research

Language(s) - English

Resource type - Journals

ISSN - 1996-0808

DOI - 10.5897/ajmr2014.6740

Subject(s) - ethyl acetate , antimicrobial , micrococcus luteus , minimum inhibitory concentration , minimum bactericidal concentration , chemistry , antibacterial activity , agar diffusion test , agar dilution , chloroform , staphylococcus aureus , agar , microbiology and biotechnology , chromatography , food science , traditional medicine , escherichia coli , bacteria , biology , biochemistry , organic chemistry , medicine , genetics , gene

The objective of this study was to collect information about medicinal plant Lonicera lanceolata. The antimicrobial assay of four fractions (n-hexane, chloroform, ethyl acetate and ethanol) obtained from Lonicera lanceolata were screened against two human Gram-positive (Staphylococcus aureus, Micrococcus luteus) and four Gram-negative pathogen (Escherichia coli, Pseudomonas aeruginosa, Enterobacter cloacae, Klebsiella pneumoniae). The antimicrobial and antifungal activities were performed by Agar well diffusion method. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by Agar well dilution method and Viable cell count method respectively. Ethyl acetate fraction exhibited maximum antibacterial activity while the other fractions that is, hexane, chloroform, ethanol also showed standard antibacterial activities. MIC and MBC were determined for ethyl acetate fraction. MICs ranged from 0.312 to >10 µg/ml and MBCs from 0.260 to 15 µg/ml. The ethyl acetate and chloroform fractions showed excellent antifungal activity while other fractions have least antifungal activity. Key words: Lonicera lanceolata, antimicrobial activity, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC).

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