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Brevundimonase diminuta as an opportunistic environmental bacterium, due to the increase in isolation rate from clinical specimens and its antibiotic resistance is considered as a new threat to human health care. After performing conventional phenotypic methods and molecular approaches, antibiotics resistance pattern on the identified Brevundimonase isolates was performed as minimum bactericidal concentration by disk diffusion method. Out of 1084 nasopharyngeal specimens, 2/1084 (%0.18) Brevundimonase diminuta, and 1/1084 (%0.09) Brevundimonase subvibrioides were isolated. Evaluation of the resistance pattern from isolated Brevundimonase strains indicated that: levofloxacin, ciprofloxacin, ampicillin, amikacin (Excluding B. subvibrioides), chloroamphenicol and ceftazidime had poor susceptibility results. Also azythromycin, gentamycin, sulfamethoxazole/trimethoprim (Excluding B. subvibrioides), ciprofloxacin, kanamycin showed good susceptibility results against Brevundimonase isolated strains. In conclusion, Although, Brevundimonase diminuta rarely has been isolated from other clinical specimens, but, due to reports of resistance to some of antimicrobial agents in this genus all laboratories should be equipped for the identification and evaluation of susceptibility patterns of this species. It is first the report of isolated Brevundimonase diminuta from nasopharyngeal specimen from suspected patients to pertussis in Iran.       Key words: Brevundimonase diminuta, nasopharyngeal specimens, 16srRNA primer.

african journal of microbiology research

First report of isolation and identification of Brevundimonase (Pseudomonas) diminuta from collected nasopharyngeal specimens in suspected patients to pertussis