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Brucella abortus and Brucella melitensis are the causative agents associated with brucellosis among cattle and buffaloes, respectively. NRAMP1 and iNOS are the best characterized genes conferring resistance against several intracellular organisms. The present study was undertaken to analyze transcript abundance of NRAMP1 and iNOS genes among Brucella sero-reactive cattle and buffalo. Our studies revealed that seropositive animals have significantly (P<0.05) higher mRNA transcript expression of both genes as compared to seronegative animals. Therefore, the present study shows that both NRAMP1 and iNOS genes are important candidates for the host response to Brucella among cattle and buffalo.   Key words: Bovine brucellosis, NRAMP1, iNOS.

Transcript abundance of bovine NRAMP1 and iNOS genes among Brucella sero-reactive cattle and buffalo