Assessment of five phenotypic tests for detection of methicillin-resistant staphylococci in Cotonou, Benin | Zendy

Dissou Affolabi | Zendy; Mathieu Odoun | Zendy; Lucrèce Ahovègbé | Zendy; Prudence Wachinou Ablo | Zendy; Kehinde Aderemi | Zendy; Sogbo Frederic | Zendy; Fahun Frank | Zendy; Bekou Kossi Wilfried | Zendy; Massougbodji Achille | Zendy; A Severin | Zendy

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Assessment of five phenotypic tests for detection of methicillin-resistant staphylococci in Cotonou, Benin

Author(s) -

Dissou Affolabi,

Mathieu Odoun,

Lucrèce Ahovègbé,

Prudence Wachinou Ablo,

Kehinde Aderemi,

Sogbo Frederic,

Fahun Frank,

Bekou Kossi Wilfried,

Massougbodji Achille,

A Severin

Publication year - 2014

Publication title -

african journal of microbiology research

Language(s) - English

Resource type - Journals

ISSN - 1996-0808

DOI - 10.5897/ajmr2014.6604

Subject(s) - cefoxitin , agar , agar diffusion test , microbiology and biotechnology , sccmec , staphylococcus aureus , biology , veterinary medicine , methicillin resistant staphylococcus aureus , medicine , bacteria , genetics

spp. isolates in Cotonou, Benin. Isolates consecutively collected from various specimens from four medical laboratories in Cotonou from December 2012 to April 2013 were included in the study. The isolates were subjected to five phenotypic tests: disk diffusion tests with cefoxitin (Cefox) and moxalactam (Moxa) on Mueller Hinton agar incubated at 37°C, oxacillin on Mueller Hinton agar incubated at 30°C (Oxa30), oxacillin on salt Mueller Hinton agar incubated at 37°C (Oxa37) and agglutination test for PBP2a detection (TPBP 2a). Results were compared with polymerase chain reaction (PCR) of

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