Production and purification of polyclonal antibodies against 34 kDa protein (P34) of Mycobacterium avium subsp. Paratuberculosis | Zendy

Alizadeh Hafezeh | Zendy; Madani Rasool | Zendy; Kavid Narges | Zendy; Golchinfar Fariba | Zendy; Emami Tara | Zendy

Open Access

Production and purification of polyclonal antibodies against 34 kDa protein (P34) of Mycobacterium avium subsp. Paratuberculosis

Author(s) -

Alizadeh Hafezeh,

Madani Rasool,

Kavid Narges,

Golchinfar Fariba,

Emami Tara

Publication year - 2014

Publication title -

african journal of microbiology research

Language(s) - English

Resource type - Journals

ISSN - 1996-0808

DOI - 10.5897/ajmr2013.6590

Subject(s) - polyclonal antibodies , paratuberculosis , antibody , antigen , mycobacterium , blot , microbiology and biotechnology , biology , western blot , virology , bacteria , immunology , biochemistry , gene , genetics

Paratuberculosis caused by Mycobacterium avium subsp. paratuberculosis (MAP), is a chronic enteritis in ruminants. Among molecular components of MAP, protein P34 was identified as specific and immunodominant. Here, we describe the production of polyclonal antibodies with defined specificity for P34. Polyclonal antibodies were generated from New Zealand white rabbit. Animals were immunized at a certain time period with purified P34, MAP antigens and Freund’s adjuvant. Antibodies were purified from serum by ion exchange chromatography. Western blotting analysis was used for evaluation of interaction between 34 kDa protein and antibodies. Key words: 34 kDa antigen, Paratuberculosis, Polyclonal antibodies.

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