Lentiviral transgenesis of the leopard gecko, Eublepharis macularius | Zendy

Hull Kaitlyn | Zendy; Hodgson Dee | Zendy; Clark Bob | Zendy; W. Hickok Timothy | Zendy; ND James | Zendy; Elahna Paul | Zendy

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Lentiviral transgenesis of the leopard gecko, Eublepharis macularius

Author(s) -

Hull Kaitlyn,

Hodgson Dee,

Clark Bob,

W. Hickok Timothy,

ND James,

Elahna Paul

Publication year - 2014

Publication title -

african journal of microbiology research

Language(s) - English

Resource type - Journals

ISSN - 1996-0808

DOI - 10.5897/ajmr2013.6532

Subject(s) - transgenesis , gecko , transgene , biology , population , microbiology and biotechnology , gene , genetics , reproductive technology , zoology , embryogenesis , medicine , environmental health

Lentiviral vectors are an effective method of introducing transgenes into the genome of early stage embryos because they transduce both dividing and non-dividing cells. Lentiviral pseudoparticles containing the coding sequence for the fluorescent protein DsRed were injected into freshly laid leopard gecko eggs. Tissue samples were collected from hatchlings, and the samples were tested for the presence of the transgene. Of the injected gecko population, greater than 89% of efficiency of transgenesis was confirmed using polymerase chain reaction (PCR). Histological evaluations revealed the presence of DsRed 2 in injected gecko organs; with protein production concentrated in the muscle, kidney, and heart. Therefore, lentiviral vectors appear to be viable technology to create transgenic geckos. Key words: DsRed, Eublepharis macularius, Feline Immunodeficienty Virus (FIV), lentiviral transgenesis, reptiles.

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