The antibacterial effect of phyto-mediated silver nanoparticles produced from Ocimum sanctum L. (Lamiaceae) leaf extract on textile fabrics

The Ocimum sanctum (holy thulsi) leaves extract perform as a reducing and capping agent in the formation of silver nanoparticles. A UV-VIS spectrum of the silver nanoparticles showed a peak at 421 nm. The morphology and size of the silver nanoparticles were carried out by the transmission electron microscope (TEM) and scanning electron microscope (SEM). The sizes of the synthesized silver nanoparticles were found to be in the range of 7 to 45 nm. The structural characteristics of biomo-lecules hosted silver nanoparticles were studied by X-ray diffraction. The chemical composition of elements present in the solution was determined by energy dispersive spectrum. The FTIR analysis of the nanoparticles indicated the presence of proteins, which may be acting as capping agents around the silver nanoparticles. This study reports that green synthesis is medicinally useful nanoparticles to avoid toxic chemicals with adverse effects in medical applications rather than physical and chemical methods. The antibacterial activity of phyto mediated silver nanoparticles was assessed by the paper disc method against Klebsiella pneumonia and 12 mm clear zone was observed. The antibacterial activity of the finished fabrics was assessed quantitatively by reduction test. The topographical analysis of the treated fabric and untreated fabric was studied and compared. The results show that the finished fabric demonstrated significant antibacterial activity against Escherichia coli.   Key words: Silver nanoparticles, antimicrobial activity, cotton, green synthesis.