Effect of Pediococcus sp., Pediococcus pentosaceus, inulin and fulvic acid, added to the diet, on growth of Oreochromis niloticus
Author(s) -
Abraham Cota Gasteacute lum Luis,
Luna Gonzaacute lez Antonio,
Abelardo Gonzaacute lez Ocampo Heacute ctor,
del Carmen Flores Mir,
a,
Arturo Fierro Coronado Jesuacute s,
Escamilla Montes Ruth,
Peraza Goacute mez Viridiana
Publication year - 2013
Publication title -
african journal of microbiology research
Language(s) - English
Resource type - Journals
ISSN - 1996-0808
DOI - 10.5897/ajmr2013.6369
Subject(s) - inulin , pediococcus , oreochromis , lactic acid , probiotic , food science , chemistry , bacteria , fermentation , biology , lactobacillus , fish <actinopterygii> , fishery , genetics
Lactic acid bacteria (LAB) were isolated from the intestine of Oreochromis niloticus and its effect, along with that of inulin and fulvic acid, on growth of the same species was determined. Characterization of the isolates was performed through assessing the Gram stain, morphology, cell arrangement, hemolytic activity, antagonism, growth, hydrophobicity, extracellular enzyme activity, and counting of colony forming units (CFU), to select those microorganisms with probiotic potential. The selected isolates were identified at the molecular level by the amplification of 23S and 5S ribosomal genes. Treatments were performed in triplicate in plastic tanks with 600 l of filtered fresh water and 12 fish per tank (1.3 ± 0.12 g):
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