Evaluation of Francis media for extended spectrum beta lactamase (ESBL) screening | Zendy

Roszilawati Ramli Siti | Zendy; Hashim Rohaidah | Zendy; Lourdes Francis Alex | Zendy

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Evaluation of Francis media for extended spectrum beta lactamase (ESBL) screening

Author(s) -

Roszilawati Ramli Siti,

Hashim Rohaidah,

Lourdes Francis Alex

Publication year - 2014

Publication title -

african journal of microbiology research

Language(s) - English

Resource type - Journals

ISSN - 1996-0808

DOI - 10.5897/ajmr2013.6328

Subject(s) - citrobacter , beta lactamase , enterobacteriaceae , microbiology and biotechnology , enterobacter , escherichia coli , klebsiella pneumoniae , biology , gene , genetics

Francis media was developed for the differential screening of Burkholderia pseudomallei. It was later found to have additional function of selecting extended spectrum beta lactamase (ESBL) from Enterobacteriaceae. A total of 305 Enterobacteriaceae isolates from clinical specimens (Klebsiella spp. [191], Escherichia coli [96], Enterobacter spp. [9], Citrobacter spp. [3] and others [6]) were tested for the presence of ESBL.Out of 305, 135 were ESBL producing Enterobacteriaceae tested on Francis media for the presence of yellow colonies and haze after 24 h of incubation. Francis media revealed sensitivity and specificity of 89 and 99%, respectively in detecting ESBL producing Enterobactericeae. Key words: Screening, extended spectrum beta lactamase (ESBL), Francis media.

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