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Herein, we developed a novel assay for detection of anaerobes by polymerase chain reaction (PCR) combined with JP (junction probe). The PCR products were processed by bead-based extraction to get single strand DNA which could be used in the junction probe reaction. The experiment results demonstrate that the new assay which we call PCR-JP could detect at least 10 copies of DNA with high sensitivity and specificity which is easily operated in the elementary medical organizations and resource-limited areas.

A novel assay combined PCR with junction probe for rapid detection of anaerobes DNA