A novel assay combined PCR with junction probe for rapid detection of anaerobes DNA | Zendy

Yan Shuai | Zendy; Li Zeng | Zendy; Wenlin Li | Zendy; Haihua Qian | Zendy

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A novel assay combined PCR with junction probe for rapid detection of anaerobes DNA

Author(s) -

Yan Shuai,

Li Zeng,

Wenlin Li,

Haihua Qian

Publication year - 2013

Publication title -

african journal of microbiology research

Language(s) - English

Resource type - Journals

ISSN - 1996-0808

DOI - 10.5897/ajmr2013.2532

Subject(s) - polymerase chain reaction , dna , dna extraction , microbiology and biotechnology , chemistry , hybridization probe , real time polymerase chain reaction , biology , gene , biochemistry

Herein, we developed a novel assay for detection of anaerobes by polymerase chain reaction (PCR) combined with JP (junction probe). The PCR products were processed by bead-based extraction to get single strand DNA which could be used in the junction probe reaction. The experiment results demonstrate that the new assay which we call PCR-JP could detect at least 10 copies of DNA with high sensitivity and specificity which is easily operated in the elementary medical organizations and resource-limited areas.

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