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Changes in tRNA profiles in Sinorhizobium meliloti CCBAU 81024 during alkali-shock
Author(s) -
Yumei Li,
Genlin Wang,
Tanghui,
Liu Zhengyu,
Jianying Li
Publication year - 2014
Publication title -
african journal of microbiology research
Language(s) - English
Resource type - Journals
ISSN - 1996-0808
DOI - 10.5897/ajmr12.421
Subject(s) - transfer rna , sinorhizobium , sinorhizobium meliloti , chemistry , rna , biology , biochemistry , genetics , gene , rhizobiaceae , bacteria , symbiosis , mutant
The changes of tRNA under alkali shock by the method of stable low-molecular-weight RNA profiles was firstly reported. In Sinorhizobium meliloti CCBAU 81024, there are a clear difference profiles obtained from cells exposed to alkaline shock. In contrast, the profiles from cells under alkaline stress conditions are undistinguishable from cells cultured at pH 7. It was shown that tRNA(s) play some roles in response to environmental stimuli, and the low-molecular-weight (LMW) RNA profiles can provide an inexpensive screening technique for measuring diversification of tRNA among the different controlled samples.   Key words: tRNA, alkali shock, low-molecular-weight (LMW) RNA profile.

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