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The changes of tRNA under alkali shock by the method of stable low-molecular-weight RNA profiles was firstly reported. In Sinorhizobium meliloti CCBAU 81024, there are a clear difference profiles obtained from cells exposed to alkaline shock. In contrast, the profiles from cells under alkaline stress conditions are undistinguishable from cells cultured at pH 7. It was shown that tRNA(s) play some roles in response to environmental stimuli, and the low-molecular-weight (LMW) RNA profiles can provide an inexpensive screening technique for measuring diversification of tRNA among the different controlled samples.       Key words: tRNA, alkali shock, low-molecular-weight (LMW) RNA profile.

Changes in tRNA profiles in Sinorhizobium meliloti CCBAU 81024 during alkali-shock