Novel complex organic substrates for cultivation of bacteria like Bacillus mojavensis A21

The media containing new complex organic substrates suggested that Mirabilis jalapa tuber powder (MJTP), shrimp wastes powder (SWP) and sardinella peptone could be used as an alternative source of cheaper carbon and nitrogen substrates for production of enzymes by Bacillus mojavensis A21. The use of 40 g/L MJTP as a sole carbon and nitrogen sources resulted in a proteases and α-amylases production of 730 and 3 U/ml, respectively. A two fold higher production of proteases by A21 strain was achieved with 4 g/L of sardinella peptones added in the MJTP medium. More interestingly, SWP supplementation in a medium containing of MJTP resulted in maximum growth and highest α-amylase production.