The essential amino acids requirements for Oenococcus Oeni growth and organic acids metabolism

Lactic acid bacteria are characterized by numerous nutritional requirements. The influence of amino acids on the growth of Oenococcus oeni SD-2a and metabolism of organic acids were determined. The microorganism in basal medium grew to 2.47×108CFU·ml-1 biomass with maximum growth rate of 0.0072 h-1. When glutamic acid and cysteine were independently omitted from basal medium, no growth was observed, indicating that these amino acids are essentials for bacterial growth. When L-malate and citric acids were added individually or together to basal media deficient in glutamic acid or cysteine, they played a beneficial role in bacterial growth and L-malic acid utilization; and both acid combined had a stimulatory effect on the rate of growth and L-malic acid utilization. When glutamic acid or cysteine concentration increased in the same media, bacterial maximum growth rate (μmax) and final biomass production increased; the rate of L-malate acid utilization, L-lactic acid production or citric acid consumption also increased.   Key words: Oenococcus oeni, amino acids, organic acids.