Sugars waste, an alternative growth and complete medium for fast growing Rhizobium cells

The sugar waste (molasses) was tested for various physiochemical parameters. After examination of various physiochemical parameters, growth and population count ofRhizobium meliloti MTCC-100 was monitored at different concentrations of sugar waste (10, 20, 30 and up to 100%) in terms of optical density (OD) and colony forming unit (C.F.U.). Growth and cell count of Rhizobium were highest in 10% sugar waste concentration. Growth pattern of the bacteria was observed at 10% sugar waste along with different synthetic media (tryptone yeast extract medium, rhizobium minimal medium and yeast extract medium). Growth of bacteria in 10% sugar waste was found to be superior to standard media (TY, RMM and YEM) used for Rhizobium. The important environmental parameters like pH and temperature were optimized for 10% sugar waste as growth medium. A pH of 7.0 and temperature of 28°C were found to be the most suitable for the fast growing R. meliloti MTCC-100.    Key words: Rhizobium meliloti, sugar waste, synthetic media, colony forming unit.