Open AccessRecombinant Clone ABA392 protects laboratory animals from Pasteurella multocida Serotype B
Author(s) -
Hussaini Jamal,
Nazmul Mhm,
Ahmad Mahmood,
Salmah Ismail
Publication year - 2011
Publication title -
african journal of microbiology research
Language(s) - English
Resource type - Journals
ISSN - 1996-0808
DOI - 10.5897/ajmr11.144
Subject(s) - pasteurella multocida , clone (java method) , immunogenicity , recombinant dna , virology , serotype , immunization , biology , cloning (programming) , microbiology and biotechnology , antibody , immunity , immune system , immunology , bacteria , dna , gene , genetics , computer science , programming language
In this study the potential of the previously contracted recombinant clone ABA392 derived from Pasteurella multocida serotype B to protect laboratory animal against haemorrhagic septicaemia was determined. After reconfirmation of plasmid DNA size, its stability and pathological effect of the clone, passive mice protection test and active immunization was carried out. Pooled serum sample from rats vaccinated with live and killed form of the clone was administered to mice and provided 66% protection while active immunization with the recombinant clone conferred 83% immunity to mice when challenged with lethal dose of P. multocida. ELISA results were positive for presence of antibody in serum of immunized mice. Sub-cloning of the insert ABA392 into an expression vector pQE32 was carried out to express its protein. It was found that the recombinant clone ABA392 is immunogenic and could be used as vaccine in future. Key words: Recombinant clone, immunogenicity, Pasteurella multocida serotype B.
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