Preparation of rabbit anti-Ganoderma sinensis immunomodulatory protein polyclonal antibody | Zendy

Qizhang Li | Zendy; Zheng Shengbo | Zendy; Xuefei Wang | Zendy; Bao Tingwen | Zendy; XuanWei Zhou | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Preparation of rabbit anti-Ganoderma sinensis immunomodulatory protein polyclonal antibody

Author(s) -

Qizhang Li,

Zheng Shengbo,

Xuefei Wang,

Bao Tingwen,

XuanWei Zhou

Publication year - 2011

Publication title -

african journal of microbiology research

Language(s) - English

Resource type - Journals

ISSN - 1996-0808

DOI - 10.5897/ajmr10.471

Subject(s) - polyclonal antibodies , rabbit (cipher) , antibody , ganoderma , chemistry , traditional medicine , biology , ganoderma lucidum , food science , immunology , medicine , mathematics , statistics

Ganoderma sinensis immunomodulatory protein (FIP-gsi) was a new protein in fungal immunomodulatory protein (FIP) family. Based on the recombinant FIP-gsi expressed inEscherichia coli, the New Zealand white rabbits were immunized with the purity protein to prepare anti-FIP-gsi polyclonal antibody. The efficacy of polyclonal antibody was detected by ELISA and Western blot. The results showed that the anti-FIP-gsi polyclonal antibody with high efficient value and specificity has been successfully preparation, and its efficient value was 1:625,000 detected by indirect ELISA, and a special band had been observed by Western blot method. This study established a method to identify FIP-gsi by immunoblotting, and will lay a foundation for further exploring the immunologic function of FIP-gsi. Key words: Ganoderma sinensis fugal immunomodulatory proteins (FIP-gsi), polyclonal antibody, ELISA, Western blot.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research