Screening of higher yield of ethyl acetate yeast and researching on ester-producing conditions

A higher yield of ethyl acetate yeast Y1 was selected from Daqu and Xiaoqu, and the production volume of ethyl acetate was 2.152 g/l when cultured in corn saccharification mash. The strain is identified as Hansenula anomala. Then, through the single factor test to study the effects of diffident culture mediums and diffident culture methods on ester-producing, the results showed that the best esterproducing culture medium for Y1 yeast was the wheat saccharification mash. The ester yield of static culture was 40% higher when compared with shaking culture. several factors were selected to carry on the orthogonal test once more, the result indicated that the optimization plan were as follows: brix was 8°, pH was 5, temperature was 25°C, culture time was 4 days, and ethyl alcohol was 2%, total ester production of Y1 was 4.812 g/l in this condition.