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Aim: The present study was undertaken to determine the seroprevalence of mycoplasma infection and possible isolation from local chickens in Niger State, Nigeria. We have looked into this problem using a combined MG/MS ELISA kit and cultural isolation.   Methods: A total of 552 blood samples were randomly collected from exanguinated chickens for serology. Tracheal swabs were collected into screwed cap bijou bottles containing 2ml of mycoplasma broth medium.   Results: The sero prevalence of indigenous chickens from Niger State was 91.83% by MG/MS Elisa. A total of 126 swabs yielded to the growth of avian mycoplasma on mycoplasma agar.   Key words: Isolation, Mycoplasma species, Avian, indigenous chickens

african journal of cellular pathology

Isolation and serological detection of mycoplasma gallisepticum and mycoplasma synoviae using a combined mg/ms enzyme-linked immunosorbent assay kit in indigenous chickens in Niger State, Nigeria