Isolation and serological detection of mycoplasma gallisepticum and mycoplasma synoviae using a combined mg/ms enzyme-linked immunosorbent assay kit in indigenous chickens in Niger State, Nigeria | Zendy

Jabbar S. Ahmed | Zendy; Saidu Lawal | Zendy; MY Fatihu | Zendy; D G Moses | Zendy; Israel Joshua Barde | Zendy; Peterside Kumbish | Zendy; Sunday Blessing Oladele | Zendy

Open Access

Isolation and serological detection of mycoplasma gallisepticum and mycoplasma synoviae using a combined mg/ms enzyme-linked immunosorbent assay kit in indigenous chickens in Niger State, Nigeria

Author(s) -

Jabbar S. Ahmed,

Saidu Lawal,

MY Fatihu,

D G Moses,

Israel Joshua Barde,

Peterside Kumbish,

Sunday Blessing Oladele

Publication year - 2015

Publication title -

african journal of cellular pathology

Language(s) - English

Resource type - Journals

eISSN - 2636-672X

pISSN - 2449-0776

DOI - 10.5897/ajcpath15.013

Subject(s) - mycoplasma synoviae , serology , mycoplasma gallisepticum , mycoplasma , seroprevalence , isolation (microbiology) , biology , veterinary medicine , agar plate , microbiology and biotechnology , virology , medicine , antibody , bacteria , immunology , genetics

Aim: The present study was undertaken to determine the seroprevalence of mycoplasma infection and possible isolation from local chickens in Niger State, Nigeria. We have looked into this problem using a combined MG/MS ELISA kit and cultural isolation. Methods: A total of 552 blood samples were randomly collected from exanguinated chickens for serology. Tracheal swabs were collected into screwed cap bijou bottles containing 2ml of mycoplasma broth medium. Results: The sero prevalence of indigenous chickens from Niger State was 91.83% by MG/MS Elisa. A total of 126 swabs yielded to the growth of avian mycoplasma on mycoplasma agar. Key words: Isolation, Mycoplasma species, Avian, indigenous chickens

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