Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) and its usefulness in soil microbial ecological studies - A review | Zendy

Asuming-Brempong Stella | Zendy

Open Access

Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) and its usefulness in soil microbial ecological studies - A review

Author(s) -

Asuming-Brempong Stella

Publication year - 2014

Publication title -

african journal of biotechnology

Language(s) - English

Resource type - Journals

ISSN - 1684-5315

DOI - 10.5897/ajbx2013.13563

Subject(s) - reverse transcriptase , reverse transcription polymerase chain reaction , complementary dna , real time polymerase chain reaction , polymerase chain reaction , biology , messenger rna , microbiology and biotechnology , gene , genetics

The reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) is a highly specific polymerase chain reaction (PCR) method that allows one to detect very low transcription levels of functional gene(s) in soil. RT-qPCR helps us to know the active members of the microbial community, and their activities can be linked with other ecological processes in soil. If after the extraction of RNA from soil, the mRNA is converted to cDNA which is then sequenced, one would analyze directly the active members of the microbial community.

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