Partial purification of invertase from Momordica charantia (bitter melon) by three phase partitioning (TPP) method | Zendy

Kübra Belligün Nihan | Zendy; Saygıdeğer Demir Burcu | Zendy

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Partial purification of invertase from Momordica charantia (bitter melon) by three phase partitioning (TPP) method

Author(s) -

Kübra Belligün Nihan,

Saygıdeğer Demir Burcu

Publication year - 2019

Publication title -

african journal of biochemistry research

Language(s) - English

Resource type - Journals

ISSN - 1996-0778

DOI - 10.5897/ajbr2019.1033

Subject(s) - momordica , invertase , chemistry , ammonium sulfate , chromatography , enzyme , melon , enzyme assay , biochemistry , biology , horticulture , traditional medicine , medicine

The single step three-phase partitioning (TPP) method was evaluated for the purification of invertase from Momordica charantia. Optimum conditions for TPP method, that is, 70% ammonium sulfate saturation, 1:0.5 t-butanol ratio and 30 min incubation time lead to 20.28% efficiency and 10.48 fold partial purification. Total protein decreased from 34.86 mg to 0.68 mg in the homogenate, and the specific activity of the enzyme increased from 21.67 to 227.02 U/mg protein. The purified enzyme showed maximum activity in 0.3 M pH 5.0 buffer at 50°C. In conclusion, the optimized single step TPP method revealed better purification. Key words: Three-phase partitioning (TPP), invertase, Momordica charantia, enzyme purification.

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