Genetic structure of cassava populations (Manihot esculenta Crantz) from Angola assessed through (ISSR) markers | Zendy

Sandra Domingos João Afonso | Zendy; Franco Cunha Moreira Ricardo | Zendy; Carlos Alberto da Silva Lédo | Zendy; Cláudia Fortes Ferreira | Zendy; da Silva Santos Vanderlei | Zendy; António Muondo Pascoal | Zendy

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Genetic structure of cassava populations (Manihot esculenta Crantz) from Angola assessed through (ISSR) markers

Author(s) -

Sandra Domingos João Afonso,

Franco Cunha Moreira Ricardo,

Carlos Alberto da Silva Lédo,

Cláudia Fortes Ferreira,

da Silva Santos Vanderlei,

António Muondo Pascoal

Publication year - 2019

Publication title -

african journal of biotechnology

Language(s) - English

Resource type - Journals

ISSN - 1684-5315

DOI - 10.5897/ajb2018.16720

Subject(s) - genetic diversity , biology , analysis of molecular variance , loss of heterozygosity , gene flow , genotype , manihot esculenta , genetic variation , genetic variability , gene , veterinary medicine , botany , population , genetics , allele , demography , sociology , medicine

Eighteen ISSR primers (between Simple Repeated Sequences) were used to estimate the genetic diversity of Angolan cassava populations in order to identify genetic variabilities and their potential to be used in the diversification of cassava crops in Angola. In order to do so, three populations from three Angolan provinces were analyzed (Cuanza Norte, Uige and Malanje), which totaled 40 genotypes. In total, 116 bands were amplified with 93.24% polymorphism and 6.4 markers per ISSR primer, on average. Nei’s gene diversity (H) and Shannon indices (I) applied to the analyzed accessions presented values ranging from H = 0.20 to 0.24 and I = 0.29 to 0.50 which are intermediate values. The total gene diversity (HT) was 0.3113, and it showed high heterozygosity in Manihot populations; this outcome indicated that these populations presented gene variability. ANOVA showed 10.41% total variance between populations and 89.59% within them. Molecular featuring revealed gene diversity in each of the assessed populations; there was gene structuring between populations. Key words: Gene diversity, molecular markers, gene flow, polymorphism.

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