Chemical composition and antioxidant activity of Lippia alba essential oil obtained by supercritical CO2 and hydrodistillation | Zendy

L. Reyes-Solano | Zendy; P. Breksa III A. | Zendy; Benigno Valdez-Torres J. | Zendy; Miguel A. Angulo-Escalante | Zendy; Bo Jiao | Zendy

Open Access

Chemical composition and antioxidant activity of Lippia alba essential oil obtained by supercritical CO2 and hydrodistillation

Author(s) -

L. Reyes-Solano,

P. Breksa III A.,

Benigno Valdez-Torres J.,

Miguel A. Angulo-Escalante,

Bo Jiao

Publication year - 2017

Publication title -

african journal of biotechnology

Language(s) - English

Resource type - Journals

ISSN - 1684-5315

DOI - 10.5897/ajb2017.15945

Subject(s) - eucalyptol , chemistry , dpph , lippia , essential oil , supercritical fluid , supercritical fluid extraction , chromatography , abts , camphor , gas chromatography–mass spectrometry , gas chromatography , extraction (chemistry) , flame ionization detector , mass spectrometry , antioxidant , food science , organic chemistry

Lippia alba essential oils were obtained by hydrodistillation (HD) and supercritical fluid (SFE) extraction methods. These were analyzed by gas chromatography-mass spectrometry-flame ionization detector (GC-FID-MS). Antioxidant activity was tested by DPPH and ABTS methods, and total soluble phenolics (TSP) were also determined. While in the SFE extract 14 compounds were identified (mostly: myrcenone 3.4%, α-terpineol 1.0% and β-caryophyllene 2.3%), in the HD extract 17 compounds were identified (mostly: eucalyptol 15.6%, myrcenone 9.3% and Z-ocimenone 5.8%). The results showed that L. alba essential oils obtained by SFE was IC50=17.35 mg/mL and by HD was IC50 = 12.45 mg/mL in DPPH assay. L. alba has an excellent eucalyptol content. Key words: Antioxidants, bioactive compounds, supercritical fluid, green technologies.

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