Open AccessSomatic embryogenesis and plant regeneration in popcorn (Zea mays L.) inbred lines
Author(s) -
Luisa Monezi Lucena Ana,
Batista de Oliveira Eloi Ivone,
Alberto Scapim Carlos,
Aparecida Mangolin Claudete,
de Fátima P. S. Machado Maria
Publication year - 2017
Publication title -
african journal of biotechnology
Language(s) - English
Resource type - Journals
ISSN - 1684-5315
DOI - 10.5897/ajb2016.15767
Subject(s) - zea mays , somatic embryogenesis , regeneration (biology) , biology , inbred strain , somatic cell , embryogenesis , botany , agronomy , microbiology and biotechnology , embryo , genetics , gene
The objective of this study was to evaluate the production of embryogenic calluses and plant regeneration of seven popcorn inbred lines (Zea mays L.) in order to identify lines for further transference of genes to a generation of transgenic plants. The N6 medium with supplementation was used to induce calluses in immature embryos, and supplemented MS medium was used to stimulate plant regeneration of P11, P12, P15 and P18 inbreed lines. The number of regenerated plants was the highest for the P15 inbred line. The P15 popcorn inbred line represents a potential genotype for transferring plant regeneration competence to other popcorn genotypes. Key words: Maize, immature embryos, callus induction, somatic embryos, regeneration competence.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom