Histochemical GUS expression of beta tubulin promoter in transgenic tobacco | Zendy

Mubeen Hira | Zendy; Aftab Bashir | Zendy; Ameen Ayesha | Zendy; Masood Ammara | Zendy; Raza Shahid | Zendy

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Histochemical GUS expression of beta tubulin promoter in transgenic tobacco

Author(s) -

Mubeen Hira,

Aftab Bashir,

Ameen Ayesha,

Masood Ammara,

Raza Shahid

Publication year - 2017

Publication title -

african journal of biotechnology

Language(s) - English

Resource type - Journals

ISSN - 1684-5315

DOI - 10.5897/ajb2016.15625

Subject(s) - transformation (genetics) , nicotiana tabacum , agrobacterium , biology , transgene , genetically modified crops , gene , microbiology and biotechnology , expression vector , promoter , beta glucuronidase , gene expression , gus reporter system , reporter gene , beta (programming language) , genetics , recombinant dna , computer science , programming language

Agrobacterium mediated plant transformation is a useful technique for stable transformation of plants. This study aimed at stable transformation of the beta tubulin (β) gene promoter and its expression analysis by histochemical GUS assay. The beta tubulin promoter sequence was cloned in plant expression vector (pGA482) and transformed stably in tobacco through Agrobacterium mediated transformation. The stable GUS expression assays for this promoter in various tissues of Nicotiana tabacum indicated its functional importance in regulating gene expression in a constitutive manner. It was concluded that the β tubulin promoter is constitutively expressed with a strength equivalent to CaMV 2X35S promoter. The isolated promoter can be used in plant genetic engineering for crop improvement in future. Key words: Constitutive, gene expression, GUS, transformation.

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