Phylogenetic analysis of 23S rRNA gene sequences of some Rhizobium leguminosarum isolates and their tolerance to drought | Zendy

Sana Khalid | Zendy; A. Hewedy Omar | Zendy; Fattah M. El-Zanaty Abdel | Zendy

Open Access

Phylogenetic analysis of 23S rRNA gene sequences of some Rhizobium leguminosarum isolates and their tolerance to drought

Author(s) -

Sana Khalid,

A. Hewedy Omar,

Fattah M. El-Zanaty Abdel

Publication year - 2016

Publication title -

african journal of biotechnology

Language(s) - English

Resource type - Journals

ISSN - 1684-5315

DOI - 10.5897/ajb2016.15450

Subject(s) - rhizobium leguminosarum , phylogenetic tree , biology , 23s ribosomal rna , phylogenetic relationship , genetic diversity , ribosomal rna , 16s ribosomal rna , gene , phylogenetic diversity , rhizobium , phylogenetics , microbiology and biotechnology , botany , veterinary medicine , genetics , bacteria , rhizobiaceae , population , rna , symbiosis , ribosome , demography , sociology , medicine

The phylogenetic relationships among thirteen Rhizobium leguminosarum bv. viciae isolates collected from various geographical regions were studied by analysis of the 23S rRNA sequences. The average of genetic distance among the studied isolates was very narrow (ranged from 0.00 to 0.04) and the studied isolates formed two main groups based on cluster analysis. The isolates were tested for their growth on YMA medium supplemented with concentrations 10, 20 and 30% of polyethylene glycol plus control. All isolates exhibited good drought-tolerant efficiencies at 10% PEG. While most of the isolates could not tolerate up to 20% PEG, isolates of Rlv6, Rlv9, Rlv12 and Rlv13 tolerated up to 20% PEG. Keywords: Rhizobium leguminosarum , 23S rRNA gene, phylogenetic tree, diversity and drought tolerance

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