Effects of 1.84 GHz radio-frequency electromagnetic field on sperm maturation in epididymis micro-environment | Zendy

Guangzhou An | Zendy; Jing Li | Zendy; Xu Shenglong | Zendy; Tao Zhao | Zendy; Kangchu Li | Zendy; Jiajin Lin | Zendy; Yan Zhou | Zendy; Lihua Zeng | Zendy; Guozhen Guo | Zendy; Guirong Ding | Zendy

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Effects of 1.84 GHz radio-frequency electromagnetic field on sperm maturation in epididymis micro-environment

Author(s) -

Guangzhou An,

Jing Li,

Xu Shenglong,

Tao Zhao,

Kangchu Li,

Jiajin Lin,

Yan Zhou,

Lihua Zeng,

Guozhen Guo,

Guirong Ding

Publication year - 2016

Publication title -

african journal of biotechnology

Language(s) - English

Resource type - Journals

ISSN - 1684-5315

DOI - 10.5897/ajb2015.14894

Subject(s) - epididymis , sperm , alkaline phosphatase , andrology , acid phosphatase , superoxide dismutase , staining , biology , h&e stain , endocrinology , medicine , immunohistochemistry , chemistry , enzyme , oxidative stress , biochemistry , genetics

In recent years, intense interest has been drawn to the effects of radio-frequency electromagnetic field (RF-EMF) on reproduction. To explore the effects of RF-EMF on sperm maturation in epididymis microenvironment, 24 male Sprague Dawley (SD) rats were randomly divided into three exposed groups (SAR 1, 2 and 4 W/kg) and one sham group. The rats in exposed group were exposed to 1.84 GHz RFEMF for 5 days (1 h/day). After that, the rats were anaesthetized immediately and epididymis were taken out. Half of them were fixed in 4% formalin and the others were placed in tissue homogenate. The morphology of sperm and microstructure of epididymis were observed under microscope after hematoxylin eosin (HE) staining. Expression of Bin1b protein was detected by immunohistochemistry; the level of glutathione (GSH) and enzymes including superoxide dismutase (SOD), acid phosphatase (ACP), alkaline phosphatase (ALP) and disaccharidase were determined by commercial kits. It was found that, compared with sham group, the sperm morphology and microstructure of epididymis did not change obviously; similarly, there was no significant change in Bin1b protein expression and the levels of GSH, SOD, ACP and ALP in exposure group. These results suggest that 1.84 GHz RF-EMF under this experimental condition could not affect the sperm maturation in epididymis microenvironment of SD rats. Keywords: 1.84 GHz, radio-frequency electromagnetic field (RF-EMF), epididymis, sperm maturation, Bin1b

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