Detection of Pseudomonas fluorescens from broth, water and infected tissues by loop-mediated isothermal amplification (LAMP) method | Zendy

Pooja Saharan | Zendy; Joginder Singh Duhan | Zendy; Kumar Gahlawat Suresh | Zendy

Open Access

Detection of Pseudomonas fluorescens from broth, water and infected tissues by loop-mediated isothermal amplification (LAMP) method

Author(s) -

Pooja Saharan,

Joginder Singh Duhan,

Kumar Gahlawat Suresh

Publication year - 2015

Publication title -

african journal of biotechnology

Language(s) - English

Resource type - Journals

ISSN - 1684-5315

DOI - 10.5897/ajb2014.14360

Subject(s) - loop mediated isothermal amplification , pseudomonas fluorescens , isothermal process , microbiology and biotechnology , pseudomonas , pseudomonadales , pseudomonadaceae , dna , bacteria , chemistry , biology , biochemistry , genetics , physics , thermodynamics

Loop mediated isothermal amplification is rapid, highly sensitive and specifically developed method for detection of bacterial infections. AprX gene for alkaline metalloprotease of Pseudomonas fluorescens was used to design four primers and loop mediated isothermal amplification (LAMP) conditions were standardized for amplification of DNA. LAMP primers successfully amplified P. fluorescens from DNA and bacterial cells taken directly from broth, water and infected tissues with high specificity and sensitivity (10 pg) under isothermal condition at 61°C. Key words: Pseudomonas fluorescens, loop mediated isothermal amplification (LAMP), rapid, simple, specificity, sensitivity.

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