Bioepoxidation of isosafrol catalyzed by radish and turnip peroxidases | Zendy

Nunes Melo Micael | Zendy; Costa Lopes Lucas | Zendy; Dariva Cláudio | Zendy; Jonathan G. dos Santos | Zendy; María Angélica | Zendy; Marquez Alvarez Heiddy | Zendy; T. Fricks Alini | Zendy

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Bioepoxidation of isosafrol catalyzed by radish and turnip peroxidases

Author(s) -

Nunes Melo Micael,

Costa Lopes Lucas,

Dariva Cláudio,

Jonathan G. dos Santos,

María Angélica,

Marquez Alvarez Heiddy,

T. Fricks Alini

Publication year - 2015

Publication title -

african journal of biotechnology

Language(s) - English

Resource type - Journals

ISSN - 1684-5315

DOI - 10.5897/ajb2014.14342

Subject(s) - raphanus , guaiacol , brassica , peroxidase , chemistry , ammonium sulfate , catalysis , botany , enzyme , chromatography , biochemistry , biology

Peroxidases (PODs) from radish (Raphanus sativus L.) and turnip (Brassica napus L.) were extracted and precipitated with ammonium sulfate using a simple, low cost and quick method. The activities of all steps performed by the vegetable PODs were measured via guaiacol assay. The epoxidation of isosafrol, catalyzed by radish (R. sativus L.) and turnip (B. napus L.) peroxidases was conducted in 20% (v/v) aqueous ethanol solution using 30% (v/v) H2O2 as the terminal oxidant. High conversion (88%) and selectivity (>98%) were obtained after 48 h. The products of the reaction were analyzed by high resolution gas chromatography (GC) and mass spectrometry. Key words: Raphanus sativus L, Brassica napus L., peroxidase, epoxidation, isosafrol.

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