X-Chromosome short tandem repeat, advantages and typing technology review | Zendy

Hadi Hameed Imad | Zendy; Abdulhasan Kareem Muhanned | Zendy; H.H Rafid | Zendy

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X-Chromosome short tandem repeat, advantages and typing technology review

Author(s) -

Hadi Hameed Imad,

Abdulhasan Kareem Muhanned,

H.H Rafid

Publication year - 2015

Publication title -

african journal of biotechnology

Language(s) - English

Resource type - Journals

ISSN - 1684-5315

DOI - 10.5897/ajb2014.14115

Subject(s) - microsatellite , str analysis , str multiplex system , biology , locus (genetics) , genetics , polymerase chain reaction , dna profiling , allele , dna , gene

Microsatellites of the X-chromosome have been increasingly studied in recent years as a useful tool in forensic analysis. This review describes some details of X-chromosomal short tandem repeat (STR) analysis. Among them are: microsatellites, amplification using polymerase chain reaction (PCR) of STRs, PCR product evaluation, PCR product purification, separation and detection, data analysis of STR by Identity Software, locus information and allele frequencies for X-chromosomal STR in different populations (DXS101, DXS7423, DXS8377, DXS6789, DXS6807 genetic loci) and advantages of X-chromosomal micro-satellites. In forensic casework and DNA databases, polymorphism in STR is important as a forensic genetic marker. Keywords: Short tandem repeat (STR) loci, forensic, interpretation.

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