English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

The present study was focused on nisin produced by  Lactococcus lactis  subsp. lactis (Fc2 isolate).  The isolate was identified by 16S rRNA using specific universal primer. The obtained cell-free supernatant inhibits the growth of  Listeria monocytogenes, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus cereus  and  Bacillus subtilis  by using agar diffusion assay. Nisin structural gene was detected by polymerase chain reaction with nisin gene-specific primer followed by cloning in PGEM-T easy vector, transformation in E. coli JM109 and direct sequencing of the construct. The Nis gene which encoded nisin Z showed identical sequences to Nis Z in Genbank with accession number AB727286, as indicated by the substitution of asparagine residue instead of histidine at position 27. In this way, it was established that the nis Z gene for nisin Z production is widely distributed. The purified nisin by chloroform extraction was analyzed on 20% SDS-PAGE and gave sharp band at ~ 3.4 kDa. The 3 dimension structure of the purified Nisin was studied by CPHModels as pdb with chimera program.   Keywords:   Lactococcus lactis , nis cloning, 16S rRNA, chloroform extraction and SDS-PAGE   African Journal of Biotechnology , Vol 13(53) 4711-4719

Cloning of nis gene and Nisin purification from Lactococcus lactis subsp. lactis Fc2