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This study presents optimization of various lignocellulosics and alkali pretreatment for maximum cellulase production by Trichoderma virdii sp. Maximum endoglucanase (642 IU/L) and exoglucanase (187IU/L) activity was achieved with maize straw at 5% concentration. Oat hay was the most suitable agro-waste for β - glucosidase (7100 IUL -1 ) production followed by maize straw (6500 IUL -1 ). Maize straw was chosen in an effort to enhance cellulase production with 0.1 N NaOH, 0.5 N NaOH and I.0 N NaOH pretreatment. 0.1 N NaOH produced desirable results showing 2.5, 1.6, and 1.7 fold increase in endoglucanase, exoglucanase and β-glucosidase activity. This 0.1 N pretreated straw produced reducing sugars 3.5 times more than untreated straw. Keywords: Lignocellulosics, maize straw, cellulase production, Trichoderma virdii , alkali pretreatment African Journal of Biotechnology , Vol 13(31) 3426-3432

Induction and optimization of cellulases using various agro-wastes by Trichoderma virdii: Effect of alkali pretreatment