Induction and optimization of cellulases using various agro-wastes by Trichoderma virdii: Effect of alkali pretreatment | Zendy

Goyal Meenakshi | Zendy; Soni Giridhar | Zendy

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Induction and optimization of cellulases using various agro-wastes by Trichoderma virdii: Effect of alkali pretreatment

Author(s) -

Goyal Meenakshi,

Soni Giridhar

Publication year - 2014

Publication title -

african journal of biotechnology

Language(s) - English

Resource type - Journals

ISSN - 1684-5315

DOI - 10.5897/ajb2014.13656

Subject(s) - cellulase , straw , chemistry , trichoderma , food science , alkali metal , rice straw , agronomy , cellulose , microbiology and biotechnology , biochemistry , biology , organic chemistry , inorganic chemistry

This study presents optimization of various lignocellulosics and alkali pretreatment for maximum cellulase production by Trichoderma virdii sp. Maximum endoglucanase (642 IU/L) and exoglucanase (187IU/L) activity was achieved with maize straw at 5% concentration. Oat hay was the most suitable agro-waste for β - glucosidase (7100 IUL -1 ) production followed by maize straw (6500 IUL -1 ). Maize straw was chosen in an effort to enhance cellulase production with 0.1 N NaOH, 0.5 N NaOH and I.0 N NaOH pretreatment. 0.1 N NaOH produced desirable results showing 2.5, 1.6, and 1.7 fold increase in endoglucanase, exoglucanase and β-glucosidase activity. This 0.1 N pretreated straw produced reducing sugars 3.5 times more than untreated straw. Keywords: Lignocellulosics, maize straw, cellulase production, Trichoderma virdii , alkali pretreatment African Journal of Biotechnology , Vol 13(31) 3426-3432

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